Fig. 6
From: Inflammation and macrophage infiltration exacerbate adult incision response by early life injury
Macrophage depletion alleviates nociceptive and inflammatory responses in neonatal injured rats. A. Fluorescence images of macrophage immune-stained for CD11b (green) in the incision site of the neonatal injured rats received intraperitoneal injection of clodronate liposomes (nIN-IN-Cld) or control liposome (nIN-IN-Veh). Scale bar = 100 μm. B. Mechanical withdrawal threshold to von Frey test. C. Cumulative spontaneous pain score. **P < 0.01, ***P < 0.001; the significant difference between the nIN-IN-Cld and the nIN-IN-Cld groups at the indicated time points (N = 8, two-way repeated measures ANOVA with Holm-Sidak posttest). D. H&E stain of the cross sections of skin or muscle taken at the incision site showed that edema, swelling, and immune cell infiltrations enhanced by prior neonatal injury in the subsequence injured tissue are decreased after clodronate liposomes injection. E. Inflammation score was significantly reduced in nIN-IN-Cld group compared with the nIN-IN-Veh groups in both skin and muscle tissue surrounding the incision. Data are shown as mean ± SEM. ***P < 0.001 vs. nIN-IN-Veh group. (N = 5, Mann-Whitney nonparametric test)