Fig. 5
From: Inflammation and macrophage infiltration exacerbate adult incision response by early life injury
Effect of ketorolac tromethamine on incision hypersensitive and inflammatory responses in neonatal injured rats. A. Intramuscular ketorolac tromethamine (nIN-ktIN) reduced incision mechanical hypersensitivity to the von Frey test in neonatal injured rats. *P < 0.05, **P < 0.01, ***P < 0.001; significant difference between the nIN-IN and the nIN-ktIN groups; (N = 8, two-way repeated measures ANOVA with Holm-Sidak posttest). B, Samples of cross sections of the paw tissue taken near the incision site were processed with the H&E method five days after paw incision from the indicated groups. Edema, swelling, and immune cell infiltrations enhanced by prior neonatal injury in the subsequence injured tissue are decreased by ketorolac tromethamine (nIN-ktIN). Scale bar = 100 μm. C, D Examples of CD11b staining (green) in the skin or the muscle as indicated at five days after paw incision with prior neonatal injury from rats received intramuscular ketorolac tromethamine (nIN-ktIN) or saline (nIN-sIN). The morphology of microphage staining for CD11b was presented as 3x magnification in the lower right corner of each picture. Scale bar = 200 μm. E. Histograms showing the expression levels of pro-inflammatory cytokines IL-6, IL-1β, and TNFα were decreased after ketorolac tromethamine injection. *P < 0.05, significant difference between the groups (N = 5,unpaired Student t test)