Fig. 4

The M1 and M2 phenotype macrophages were increased in the adult rats with prior neonatal injury after repeat hindpaw incision. A. Cells collected from the injured hind paws from the nsham-IN and the nIN-IN groups five days after surgery were subjected to flow cytometric analysis. Dot plots circled by red boxes represent rat macrophages stained with fluorochrome-labeled specific antibodies as indicated in the nsham-IN and the nIN-IN groups. Total macrophages are represented as anti-CD45 /anti-CD11b double-labeled dot plots. Derived from total macrophages, the M1 phenotype is represented as anti-CD86 /anti-CD68 double-labeled dot plots, and the M2 phenotype is represented as anti-CD163 /anti-CD68 double-labeled dot plots. Quantifications of total (B), M1 (C), and M2 (D) phenotype macrophages showed that the population of each type of macrophage is significantly increased in the nIN-IN group. *P < 0.05, **P < 0.01, significant difference between the groups (N = 5,unpaired Student t test)