Fig. 3
From: Inflammation and macrophage infiltration exacerbate adult incision response by early life injury
Neonatal injury enhances macrophage infiltration in the injured skin after repeat hindpaw incision in the adult. A. Fluorescence images of myeloid cells immunostained for CD11b (green) in the hindpaw skin of nsham-IN and nIN-IN groups (B) Band of western blot and quantitative analysis showed the up-regulated expression of CD11b induced in the nIN-IN group. ***P < 0.001, vs. nsham-IN group (N = 6, unpaired Student t test). C. Examples of M1 phenotype macrophage marker CD 86 staining in hindpaw skin of nsham-IN and nIN-IN groups. D. Band of western blot and quantitative analysis showed the expression of CD86 increased in the nIN-IN group. **P < 0.01, vs. nsham-IN group (N = 6, unpaired Student t test). E. M2 phenotype macrophage marker CD 163 staining in the hindpaw skin of nsham-IN and nIN-IN groups (Scale bar = 200 μm). F. Band of western blot and quantitative analysis showed that the expression of CD163 increased in the nIN-IN group. *P < 0.05, vs. nsham-IN group (N = 6, unpaired Student t test)