Fig. 1
From: Inflammation and macrophage infiltration exacerbate adult incision response by early life injury
Neonatal injury enhances mechanical hypersensitivity, spontaneous pain, and microglia activation in adult rats’ spinal cord dorsal horns after a repeat hindpaw incision. (A) Mechanical withdrawal threshold to von Frey test. (B) Cumulative spontaneous pain score. **P < 0.01, ***P < 0.001; the significant difference between the nIN-IN and the nsham-IN groups at the indicated time points (N = 8, two-way repeated measures ANOVA with Holm-Sidak posttest). (C) Example sections of spinal dorsal horn immunolabeled with microglia marker Iba-1 (green) five days after paw incision. Scale bar = 200 μm. (D) Quantitative summary indicated IBA1 expressions were upregulated after repeat hindpaw incision in the ipsilateral spinal dorsal horn. **P < 0.01, ***P < 0.001 significant difference between the labeled groups. (N = 4, unpaired Student t-test). (E) The magnification of the white squares showed the hypertrophy of microglia with an enlarged, darkened soma and shorter, thicker, less branched processes after repeating the hindpaw incision. Scale bar = 50 μm