Fig. 5

Identification of neurogenesis-associated lncRNAs in aging mice. A Differential expression of lncRNAs, miRNAs, and mRNAs between aging mice within the surgery as well as the control group, with a constructed ceRNA network based on their interactions. B-E Representative lncRNAs whose downstream mRNAs are enriched in the ‘neurogenesis’ (GO:0022008) GO term. The gray pies illustrate the proportion of ‘neurogenesis’ (GO:0022008) GO term-related genes (dark color) in the genome. The orange, cyan, purple, and pink pies represent the proportions of mRNAs (darker color), downstream of four different lncRNAs and enriched in GO:0022008 term among all downstream mRNAs of the respective lncRNA. F-I Expression of NONMMUT062908.2, NONMMUT069618.2, NONMMUT137220.1, and NONMMUT029772.2 was verified within the aging control as well as the aging surgery group using RT-qPCR (n = 6). J-N The validation findings of miR-6976-3p, miR-6985-5p, Arpp19, Fbxl2 and Nek7 by RT-qPCR. *P < 0.05 contrasted with the control group, **P < 0.01 contrasted with the control group, ***P < 0.001 compared to the control group