Figure 2

Effects of isoflurane on H/R-induced apoptosis shown by TUNEL labeling. Top panel, representative images; Bottom panel, quantification of fluorescence intensity (cells were randomly selected from different areas; each value was from three separate experiments). Con, control (n = 34); H/R, hypoxia/reoxygenation (n = 38); Iso + H/R, isoflurane plus H/R (n = 40); Iso, isoflurane alone (n = 36). *p < 0.01, compared to the control group; # p < 0.01, compared to the H/R group. Scale bar, 50 μm.